Fig. 4: Urea cycle dysregulation in neuronal cell models of PD.
From: Urea cycle dysregulation drives metabolic stress and neurodegeneration in Parkinson’s disease
A Western Blot analysis of key urea cycle enzymes and urea channel proteins in PC12, SH-SY5-Y and Neuro-2a MPTP model. B RNA relative expression of ODC1. C Measurement of urea, ammonia and putrescine level changes in the MPTP model. n = 3. D Western Blot analysis of urea cycle key enzyme and urea channel protein expression in neurons induced by high urea. E Western Blot analysis of the relative expression of key urea cycle enzymes and urea channel proteins in neurons induced by high urea. n = 4. F Western Blot analysis of key urea cycle enzymes and urea channel proteins in C8-D1A astrocytes. G Measurement of putrescine level changes after urea treatment. Data are presented as the mean ± SEM. Significant differences were established by Student’s t-test. ***p < 0.001, **p < 0.01, and *p < 0.05.
