Table 2 Statistical results from the Jonckheere–Terpstra test for concentration-dependent analysis of proteins enriched in the top 5 densely connected modules originally identified at the high-concentration MGO-treated group (1000 μM MGO)

From: An integrated proteomics and metabolomics analysis of methylglyoxal-induced neurotoxicity in a human neuroblastoma cell line

No.

Protein (gene name)

Fold change (FC)

J statistica

p value

Concentration-dependent change

Low

Medium

High

1

EIF3C

1.07

1.17

1.23

3.92

0.0001

↑

2

PES1

1.10

1.46

1.56

3.74

0.0002

↑

3

HMOX1

1.19

3.03

6.38

3.69

0.0002

↑

4

UTP4

1.13

1.53

1.73

3.55

0.0004

↑

5

EIF3D

1.05

1.19

1.28

3.55

0.0004

↑

6

BRIX1

1.24

1.39

1.51

3.46

0.0005

↑

7

RBM28

1.03

1.32

1.37

3.27

0.0011

↑

8

EIF4A1

1.12

1.12

1.50

3.27

0.0011

↑

9

PDCD11

0.92

1.21

1.27

3.18

0.0015

↑

10

EBNA1BP2

1.04

1.36

1.35

3.08

0.0021

↑

11

WDR75

1.00

1.70

9.36

3.08

0.0021

↑

12

BOP1

1.10

1.44

1.42

2.99

0.0028

↑

13

MRTO4

1.10

1.27

1.34

2.99

0.0028

↑

14

ESF1

1.46

2.74

4.62

2.83

0.0046

↑

15

PPAN

1.04

1.56

1.50

2.80

0.0051

↑

16

EIF3I

1.05

1.07

1.31

2.80

0.0051

↑

17

EIF3J

1.14

1.40

1.35

2.80

0.0051

↑

18

NQO1

1.32

1.40

1.56

2.80

0.0051

↑

19

DDX27

1.02

1.49

1.43

2.71

0.0068

↑

20

EIF1AX

1.16

1.06

1.52

2.71

0.0068

↑

21

EIF4G1

0.99

1.27

1.24

2.61

0.0089

↑

22

DDX47

1.28

1.35

1.57

2.52

0.0117

↑

23

EIF2S1

1.20

1.06

1.27

2.52

0.0117

↑

24

TBL3

1.11

1.05

1.23

2.43

0.0152

↑

25

EIF1

0.92

1.13

1.37

2.43

0.0152

↑

26

UQCRB

1.04

1.11

1.26

2.33

0.0196

↑

27

TXNRD1

1.07

1.09

1.45

2.33

0.0196

↑

28

NDUFS4

1.00

9.97

4.48

2.17

0.0302

↑

29

EIF3M

1.30

1.04

1.59

2.05

0.0399

↑

30

EIF2S3

1.11

0.97

1.25

1.96

0.0499

↑

31

FLNC

1.15

1.04

1.25

1.96

0.0499

↑

32

NDUFB4

0.85

1.03

0.74

−1.96

0.0499

↓

33

RPS27

0.88

0.94

0.81

−2.15

0.0317

↓

34

ITGA3

0.97

0.94

0.82

−2.43

0.0152

↓

35

COL1A1

0.89

0.89

0.40

−2.61

0.0089

↓

36

ITGAV

1.03

0.87

0.72

−2.61

0.0089

↓

37

NDUFA7

0.33

0.39

0.03

−2.64

0.0083

↓

38

ITGA6

0.68

0.79

0.56

−2.71

0.0068

↓

39

COX5B

0.79

0.80

0.68

−2.80

0.0051

↓

40

TLN2

0.93

0.76

0.74

−2.89

0.0038

↓

41

LAMB1

0.97

0.80

0.69

−3.18

0.0015

↓

42

ITGB1

0.95

0.87

0.80

−4.11

0.00004

↓

43

RPS12

1.12

0.98

1.21

1.87

0.0618

 

44

EIF3L

1.15

0.96

1.31

1.87

0.0618

 

45

WDR3

1.13

0.68

1.48

1.77

0.0760

 

46

PWP2

1.17

0.68

1.25

1.77

0.0760

 

47

EIF6

1.25

0.86

1.70

1.68

0.0928

 

48

EIF3H

1.10

0.75

1.34

1.68

0.0928

 

49

RPS26

1.10

1.04

1.26

1.59

0.1124

 

50

TXN

1.28

1.23

1.39

1.59

0.1124

 

51

RPS29

0.78

1.20

0.83

−1.31

0.1911

 

52

GCLM

1.00

1.75

9.93

1.12

0.2624

 

53

ATP5F1D

1.69

0.38

1.54

1.07

0.2824

 

54

NOB1

0.92

1.21

0.76

−1.03

0.3043

 

55

RPL32

0.85

1.19

0.79

−1.03

0.3043

 
  1. Proteins were indicated by their corresponding gene names, which were retrieved from UniProt38 “↑” indicates a significant concentration-dependent increase in the expression levels with increasing MGO concentrations (p < 0.05), while “↓” indicates a significant concentration-dependent decrease (p < 0.05).
  2. aThe J statistic values are ranked in descending order for the proteins with p < 0.05 and reflect the direction and magnitude of the trend in the protein expression levels with increasing MGO concentrations. A positive J statistic value indicates an upward trend, suggesting that the protein levels increase as MGO concentration rises. A negative J statistic value indicates a downward trend, suggesting protein levels decrease as MGO concentration rises.
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