Fig. 3: Recombinant COR15B and COR47 expressed heterologously in E. coli exhibit antifreeze activity. | npj Science of Food

Fig. 3: Recombinant COR15B and COR47 expressed heterologously in E. coli exhibit antifreeze activity.

From: Identification of ice-binding proteins from Raphanus sativus and application in frozen dough

Fig. 3

A PCR detects positive clones in BL21. M: DNA marker DL5000; Monoclonal strain 1-3. B Expression and purification proteins assayed by coomassie blue staining. Input: E. coli lysate; Elution: solution adsorbed with His beads. Arrows point the target proteins. C Ice crystal morphology modification activity of purified COR15B and COR47. Hexagonal ice crystals are highlighted in red. D The purified proteins were detected by western blot. E, F Recrystallization inhibition activity assayed and the ice crystal area distribution in different concentrations of COR15B and COR47. Blank: ddH2O.

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