Fig. 2: Analyses of cell populations simulated by the cell cycle progression model.
a Growth curve, expressed as number of cells per 0.58 mm2, obtained for two simulated NIH3T3 populations followed for 15 days while growing at 10% (grey) and 2.5% (blue) calf serum, and for a third population (green) initially kept at 10 calf serum and moved to 2.5% on the sixth day (arrow). b Cell fractions in the different cycle phases on the 3rd day (exponential growth) and on the 15th day (stationary state). c Growth curve, expressed as number of cells per 0.58 mm2, for four simulated NIH3T3 populations seeded at four different densities (1, 4, 10, 50 per 0.58 mm2) and followed for 15 days while growing at 5% calf serum (grey). d Percentage of cells in the different cycle phases on the 3rd and 15th day. e Growth arrest of simulated population induced by serum starvation and rescue by serum re-addition. NIH3T3-like cells were simulated while growing under 10% FBS and, at day 1, starved by moving them to 0.5% FBS; on day 4 serum was restored to 10% and cells were followed for 3 more days. Total number of cells (blue line) and the number of cells in S (green line) and G0 (yellow line) phase are shown in the plot. f Snapshots of the simulated population in (e) at day 1, 3, 5 and 7 are reported by plotting each cell according to cdt1 and p27 concentration.
