Fig. 5: Attachment of recombinant E. coli BL21 cells expressing the antigen candidates to HBE cells. | npj Vaccines

Fig. 5: Attachment of recombinant E. coli BL21 cells expressing the antigen candidates to HBE cells.

From: Proteomic approach to identify host cell attachment proteins provides protective Pseudomonas aeruginosa vaccine antigen FtsZ

Fig. 5

A Representative images taken with the automated Opera Phenix™ confocal HSC microscope, showing the increased attachment of the induced cultures. Bacteria are shown in green (anti-E. coli antibody-FITC), and HBE cells in red (membranes, CellMask™ Deep Red) and blue (nuclei, DAPI). B Number of bacteria attached per 100 HBE cells, counted automatically by the Harmony®4.8 software In99 fields. Bars represent the mean and SD of the duplicates. C Confirmation by 12% SDS–PAGE (upper) and western blot (lower) of protein expression (red arrows) only in the induced cultures (+).

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