Fig. 6: Protective effect of immunisation with rFtsZ and rOpmH plus SAS in mice.
A Timeline of the immunisation schedule, indicating days of immunisation, blood collection, challenge, and organ harvest. Mice were immunised three times subcutaneously with 50 µg rFtsZ or rOpmH plus SAS, and then challenged with P. aeruginosa KK1 strain (6.3 × 106–1.8 × 107 CFU/mouse). B Bacterial burden in the lungs and spleens of mice 24 h post-challenge. C Severity score of mice 24 h after challenge. These graphs show data pooled from two independent experiments. Data are represented as mean ± SEM, and each point represents one mouse. Statistically significant differences were calculated using the non-parametric Kruskal–Wallis test or One-way ANOVA test (p value < 0.05). D Serological analysis of antigen-specific total IgG, IgG1 and IgG2c production by immunised mice. Each point represents the mean ± SD of the mice in the group. The grey, dash lines show the cut-off (2 SD + mean) for antibody titration. Significant differences between control and immunised groups were analysed via two-way ANOVA Šidák’s multiple comparison test (p value < 0.05) (*p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001; ****p ≤ 0.0001).
