Fig. 7: Cellular profiles of antigen-specific immune response. | npj Vaccines

Fig. 7: Cellular profiles of antigen-specific immune response.

From: Proteomic approach to identify host cell attachment proteins provides protective Pseudomonas aeruginosa vaccine antigen FtsZ

Fig. 7

Splenocytes from mice immunised (I) with either rFtsZ (green triangles), rOpmH (red squares) or adjuvant only controls (C, blue triangles and squares) were stimulated ex vivo with rFtsZ (triangles) or OpmH (squares) 14 days post immunisation and analysed by flow cytometry. Data represent percentage of cells in response to ex vivo stimulation with rFtsZ or rOpmH adjuvanted with SAS: a total T cells; b regulatory T cells; c γδ T cells; d NK T cells; e NK cells; f–j total, activated, naïve, memory or effector helper T cells; k–o total, activated, naïve, memory or effector cytotoxic T cells. The graphs represent the mean ± SD data pooled from two independent experiments and each dot represents one mouse. Statistically significant differences (p < 0.05) were evaluated using paired Student’s t-test between antigen and its respective adjuvant only control (*p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001; ****p ≤ 0.0001).

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