Fig. 8: Cytokine profile of antigen-specific immune response. | npj Vaccines

Fig. 8: Cytokine profile of antigen-specific immune response.

From: Proteomic approach to identify host cell attachment proteins provides protective Pseudomonas aeruginosa vaccine antigen FtsZ

Fig. 8

Splenocytes from mice immunised (I) with either rFtsZ (green triangles), rOpmH (red squares) or adjuvant only controls (C, blue triangles and squares) were stimulated ex vivo with rFtsZ (triangles) or OpmH (squares) 14 days post immunisation and analysed by flow cytometry. Data represent percentage of cells producing IFN-γ, TNF, IL-4, IL-17A or IL-22 in response to immunisation with rFtsZ + SAS or rOpmH + SAS. a–e non-T cells; f–j T cells; k–o helper T cells; p–r NK cells; s, t cytotoxic T cells. C control, I immunised. The graphs show data pooled from two independent experiments, which is represented as the average ± SD, and each dot represents one mouse. Statistically significant differences (p < 0.05) were evaluated using paired Student’s t-test (*p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001; ****p ≤ 0.0001).

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