Fig. 3: Serum antibodies from vaccinated mice showed binding to HA stem and head domains.
From: Use of equine H3N8 hemagglutinin as a broadly protective influenza vaccine immunogen
A We performed ELISA for endpoint titers against headless HAs (sHA2) or recombinant HA1 portions of various rHAs (n = 6 mice, 2 reps). HA-reactive antibodies were not detected in sham-vaccinated mice (not shown). p < 0.05 or less for all comparisons to controls. B We performed ELISA competition-binding assays for antigens with pooled vaccinated mice (2x) or horse (1x) sera against panels of known bnAbs. Our sera competed for binding of antibodies specific for the esterase region (mAb H3v-47), head region (mAb H3v-95), or stem region (a recombinant mAb-based on the sequence of CR9114) for binding to HA from H3N2 A/Texas/50/2012. Pools of n = 5 mice or pools of n = 10 horses were used. Antibodies used for this assay are published prior67,68,69.
