Fig. 4: Protection against infection after vaccination.
From: Use of equine H3N8 hemagglutinin as a broadly protective influenza vaccine immunogen
A We vaccinated mice as stated prior in the results twice using rHAs from either H3N8 and tested for HAI responses across many seasonal strains of influenza (n = 5 for two reps). B Next, we tested for survival after H1N1 A/California/07/2009 challenge (4 × LD50). PR8 HA was used here instead of our H1/H3 controls since they contained the H1N1 A/California/07/2009 virus (n = 5 for two reps). C–F Next, we tested for survival after the challenge with 2 × LD50 of viruses stated in the figures. Here, we compared our H3N8 whole vaccine against our H3N8 rHA and a sham or control vaccine containing H1 and H3 viruses (n = 5 for two reps for all animals). Per our animal protocol, we needed to use a 25% weight cutoff before euthanizing animals after the challenge. * p < 0.01 for each survival curve between equine vaccinees (LAIV or rHA) and both controls. The xPR8 in each challenge virus indicates these were viral crosses with A/PR/8/34 to facilitate infection in mice given its mouse adaptation.
