Fig. 7: Reduced viral shedding in H3N8 vaccinees after challenge with H1N1 A/California/07/09.
From: Use of equine H3N8 hemagglutinin as a broadly protective influenza vaccine immunogen
Ferrets rested 3 weeks post their third vaccine and were then challenged with 107 TCID50 of H1N1/Cali/09. A Ferrets were euthanized after 6 days, and lungs were stained with anti-influenza A antibody. A representative stain is shown for all groups. Brightfield images were merged with FITC images by the camera software (4× magnification). Green indicates the presence of the influenza virus. B We assessed the viral burdens in nasal swabs 3 days post-infection from the same ferrets using one-step qRT-PCR for a flu-conserved M2 region. C 1 mg of lung tissue was homogenized and filtered, and then virus titer was determined by TCID50 assay. 10× magnification was used in the images.
