Fig. 6: Positive effects of NP-N417D, M1-V219I, and/or NS1-R140W mutations on replication pathway of H9N2 AIV. | npj Vaccines

Fig. 6: Positive effects of NP-N417D, M1-V219I, and/or NS1-R140W mutations on replication pathway of H9N2 AIV.

From: Impact of inactivated vaccine on transmission and evolution of H9N2 avian influenza virus in chickens

Fig. 6

A DF1 cells were infected with rgHB17 or rgHB17(NP-N417D) at MOI of 2. NP was localized with confocal microscopy at the indicated time points (scale bar: 50 μm). B Quantitative analysis of NP localization in virus-infected cells. C H9N2 viral morphology examined with transmission electron microscopy (scale bar: 500 nm). D Quantification of spherical and filamentous virions. E Nuclear-cytoplasmic localization of wild-type HB17 and mutant NS1 protein in DF1 cells during infection. Scale bar, 10 μm. F Quantitative localization of wild-type and mutant NS1 proteins in DF1 cells.

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