Fig. 2: Staphylococcal surgical site and superficial wound infection models in minipigs. | npj Vaccines

Fig. 2: Staphylococcal surgical site and superficial wound infection models in minipigs.

From: A SpA+LukAB vaccine targeting Staphylococcus aureus evasion factors restricts infection in two minipig infection models

Fig. 2

A Modified schematic presentation of the surgical site minipig model as was described in ref. 8. B Schematic presentation of the superficial wound minipig model. A full thickness 2 cm long incision is made under anesthesia exposing the muscle layer. The challenge is released into the wound with a pipettor while moving the tip down the length of the wound. The incision remained open for the 8-days infection period. Eight days after infection the animal is euthanized and the skin from both sides of the incision is collected and homogenized. Samples were processed as described for the SSI model. Immunization schedule is shown below each picture. CFU colony-forming units, D day, IM intramuscularly, ID intradermal.

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