Fig. 3: Levels of IgGs specific against Tn and STn mimetics in serum of mice immunized with CRM-Tn or GMMA-Tn.

IgGs specific for Tn mimetic (left panel) and STn mimetic (right panel) were measured by ELISA in sera after the first immunization (post 1) and after the second immunization (post 2). BSA conjugated with derivative 16 (see Supplementary) or 2 (see Scheme 1) was used for ELISA plate coating. Data represent geometrical mean titers (GMT) of IgG titer of individual mouse sera ± SD of 3 replicates. Statistical significance was calculated by using the two-way ANOVA Tukey’s multiple comparison test; *P-value < 0.05; ****P-value < 0.0001.