Fig. 1: Selection of chimeric spike antigens with broad neutralising activity.
A Neutralisation of pseudoviruses by plasma from mice immunised with CSA candidates. Mice were vaccinated twice, 3 weeks apart with 5 μg of each spike antigen formulated in SWETM adjuvant. Twenty-one days after the last dose, nAb titres against pseudotyped viruses was determined. Grey bars indicate data points not determined. B Area under the curve (AUC) to demonstrate breadth of nAbs across different variants. Phylogenetic difference is based on variant RBD amino acid sequence. The results are expressed as the number of amino acid substitutions per 100 amino acids, relative to ancestral virus. C Individual plots for AUC analysis. Spk spike, Ancstrl Ancestral.
