Fig. 4: G129-vaccinated mice elicit a specific T cell immune response in the 20 and 50 µg groups when compared to the control group (EDIII-only). | npj Vaccines

Fig. 4: G129-vaccinated mice elicit a specific T cell immune response in the 20 and 50 µg groups when compared to the control group (EDIII-only).

From: A VLPs based vaccine protects against Zika virus infection and prevents cerebral and testicular damage

Fig. 4

Flow cytometry was performed on splenocytes from EDIII-QβVLPs (20 and 50 µg) or EDIII-only at 28 dpi. For cytokines detection, splenocytes were restimulated in vitro with EDIII protein in the presence of brefeldin A for 4 h. a Representative dot plot and frequency of CD38+ (activation T cells) among CD3+. b Representative dot plot and frequency of CD62L- (effector) among CD3+. c Representative counter plot and frequency of IL-2, IL-4, and TNF-∝ among CD4+ T cells. d Representative counter plot and frequency of IL-2, and TNF-∝ among CD8+ T cells. All graphs show the distribution of values using boxplots, where the central line represents the median, the boxes represent the interquartile range (25th–75th percentiles), and the whiskers indicate the range of the data. The number of animals per group was: only EDIII-20 µg (n = 8), EDIII-QβVLP-20 µg (n = 10), and EDIII-QβVLP-50 µg (n = 10). Data are representative of three independent experiments. Data were analyzed by Kruskal–Wallis, and post-hoc by Dunn. * p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001; **** p ≤ 0.0001.

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