Fig. 5: Fluaxe vaccination protected mice from multiple influenza virus infections.
From: Chimeric hemagglutinin and M2 mRNA vaccine for broad influenza subtype protection
A Schematic diagram of mice immunization and influenza virus challenge. Created in BioRender. Li (2025) https://BioRender.com/bithl81. Prime-boost vaccination was executed on Days 0 and 14. Four influenza subtypes (10LD50) were used to challenge mice via intratracheal administration. Survival rate, weight loss, viral RNA in lung, and pathologic level were measured during 14 days post infection. The survival rate and weight loss of Fluaxe vaccinated mice were monitored post-challenge with B A/PR/8/1934 (H1N1) virus, D A/Aichi/2/1968 (H3N2) virus, F B/Brisbane/60/2008 (IBV/Victoria) virus, and H B/Phuket/3073/2013 (IBV/Yamagata) virus, respectively. Weight loss data are exhibited as Mean ± SD. The dotted lines in weight loss figures indicate the maximum body weight loss (25%) for the experiment. Kaplan–Meier survival curves of mice are shown, and significance testing was done by log-rank test (**p < 0.01). Viral RNA (vRNA) load in lungs of Fluaxe vaccinated mice were monitored post-challenge with C A/PR/8/1934 (H1N1) virus, E A/Aichi/2/1968 (H3N2) virus, G B/Brisbane/60/2008 (IBV/Victoria) virus, and I B/Phuket/3073/2013 (IBV/Yamagata) virus on Day 5 post-infection. Data of viral RNA are shown as Mean ± SEM (n = 5 per group) and significance was calculated using two-tailed unpaired t-tests (***p < 0.001), data are normalized to the LNP group, with the control value arbitrarily set to 1.
