Fig. 1: Flowchart showing the overall DNA production, upstream, and downstream processes for the expression and purification of 16055 DG4 NFL trimer. | npj Vaccines

Fig. 1: Flowchart showing the overall DNA production, upstream, and downstream processes for the expression and purification of 16055 DG4 NFL trimer.

From: Accelerated cGMP production of near-native HIV-1 Env trimers following electroporation transfection and immunogenicity analysis

Fig. 1

16055 DG4 NFL plasmid DNA was produced from an expanded culture from an E. coli MCB. Plasmid DNA suitable for use in GMP was purified in a two-column chromatography process using Aldevron’s GMP-Source™ service (left panel). 16055 DG4 NFL was produced in CHO-S using a MaxCyte® VLX™ transfection system under GMP standards (middle panel). 16055 DG4 NFL trimers were purified from the clarified media harvest using a four-step chromatography method. Purification includes an immunoaffinity capture step using bNAb PGT145 to recover properly folded trimers, followed by three additional chromatography and further polishing steps (right panel).

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