Fig. 2: Upstream production process for large-scale expression of the 16055 DG4 NFL trimer using electroporation-based transient transfection. | npj Vaccines

Fig. 2: Upstream production process for large-scale expression of the 16055 DG4 NFL trimer using electroporation-based transient transfection.

From: Accelerated cGMP production of near-native HIV-1 Env trimers following electroporation transfection and immunogenicity analysis

Fig. 2

The high-density cell slurry was mixed with 16055 DG4 NFL plasmid DNA and electroporated using duplicate MaxCyte® VLX™ process assemblies. Transfected cells were rested in cell factories then expanded to 5 × 106 cells/mL in 63 L of production media. Fed batch culture was maintained for eight days, and media was harvested for downstream purification.

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