Fig. 3: 16055 DG4 NFL engineering run trimer displayed favorable biophysical properties. | npj Vaccines

Fig. 3: 16055 DG4 NFL engineering run trimer displayed favorable biophysical properties.

From: Accelerated cGMP production of near-native HIV-1 Env trimers following electroporation transfection and immunogenicity analysis

Fig. 3

A Analytical SEC was performed using a Superdex 200 Increase column. B Blue-native PAGE (BN-PAGE). Uncropped gel image is shown in Supplemental Fig. 1c. C 2D class averages of the major trimer peak by negative stain EM. D DSC analysis showed that the trimer melts at 75 °C as a single peak. E The trimer displayed a favorable antigenic profile as determined BLI and binds to a panel of bNAbs targeting selected cross-neutralizing epitopes on the trimer from the gp120 apex toward the gp41 base. F Minimal binding was observed with a panel of non-bNAbs.

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