Fig. 4: CryoEM reconstruction statistics and final model. | npj Vaccines

Fig. 4: CryoEM reconstruction statistics and final model.

From: Accelerated cGMP production of near-native HIV-1 Env trimers following electroporation transfection and immunogenicity analysis

Fig. 4

A Fourier Shell Correlation resolution estimate. B local resolution estimation (in units Å) C angular distribution of observations. D Cryo-EM structure of 16055 DG4 NFL trimer with protein design elements highlighted. The three monomers comprising the trimer are shown as pink, green and gray ribbons. Protein design elements include: intra-protomer disulfide bridge I201C-A433C (CC) shown in yellow, trimer-derived (TD) substitutions shown as blue balls, proline and glycine substitutions in gp41 shown as magenta balls, asparagine to glutamine (N to Q) substitutions in the CD4 binding site shown as green balls (glycan knockouts, KO), and gp120 V3 and gp41 fusion peptide (V3-FP) mutations shown in red.

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