Fig. 1: Design and characterization of mRNA-LNPs. | npj Vaccines

Fig. 1: Design and characterization of mRNA-LNPs.

From: A multivalent mRNA-LNP cocktail vaccine confers superior efficacy against Staphylococcus aureus infection in murine models

Fig. 1

A The maximum likelihood phylogenetic tree illustrating the amino acid sequence identity of the antigens (MntC, SEB, Hla, FnBPA, and IsdB) encoded by the mRNA constructs, compared to those from 99 S. aureus strains, was created using Parsnp. B Schematic of five mRNA constructs used in this study. C Capillary electrophoresis image displaying the quality and purity of in vitro synthesized mRNAs. D Particle size distribution of mRNA-LNPs as detected by Malvern particle size analyzer, data presented as average particle diameter. PDI Polydispersity Index. E Average encapsulation efficiency of mRNA-LNPs. F Western Blot analysis showing protein expression in 293T and J774A.1 cells 48 h post-transfection with mRNA-LNPs. NT, no-treatment. G CCK-8 assay evaluating the viability of HEK293T cells 72 h post-transfection with mRNA-LNPs. H SDS-PAGE analysis showing the purity of purified target proteins. Data in (D, E, G) were presented as mean ± SD, n = 3.

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