Fig. 1: Comparison of protein expression induced by unmodified and modified Sing16 HA mRNA formulated with LNPs in HSKM and hDC cells. | npj Vaccines

Fig. 1: Comparison of protein expression induced by unmodified and modified Sing16 HA mRNA formulated with LNPs in HSKM and hDC cells.

From: Synergistic effect of nucleoside modification and ionizable lipid composition on translation and immune responses to mRNA vaccines

Fig. 1

a Graph represents mean fluorescence intensity (MFI) of hemagglutinin (HA) protein expression of 1 μg or 10 μg of Sing16 HA mRNA-LNPs transfected per million cells, determined using IF 24 h post-transfection in primary human myoblast (HSKM) cells. b Graph represents the percentage of viable hDCs transfected with 5 μg of Sing16 mRNA-LNPs determined using flow cytometry 24 h following transfection. c Representative flow cytometry plots for determining HA protein expression in hDCs are shown. d Graph represents the percentage of Sing16 HA positive cells following transfection of hDCs with 5 μg of Sing16 HA mRNA-LNP, determined using flow cytometry 24 h post-transfection. P-value determined by unpaired T-test analysis. N = 3 experimental replicates and 6 replicates in each experimental repeat for figures a, b and d. e Graph represents mean MFI of HA protein from HSKM cells transfected with 1 μg/million cells of mRNA OF-02 LNP encoding four different strains: A/Wisconsin, A/Tasmania, B/Washington and B/Austria detected with strain-specific primary antibody. n = 3 experimental replicates with n = 8 replicates for each experimental repeat. P-values were determined by unpaired T-test analysis. N = 3 independent donor samples. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001.

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