Fig. 6: Serum cytokine evaluation of UNR vs. MNR mRNA-LNP vaccines in mice and non-human primates. | npj Vaccines

Fig. 6: Serum cytokine evaluation of UNR vs. MNR mRNA-LNP vaccines in mice and non-human primates.

From: Synergistic effect of nucleoside modification and ionizable lipid composition on translation and immune responses to mRNA vaccines

Fig. 6

a BALB/c mice (n = 8 per group) were immunized twice intramuscularly, 3 weeks apart with 1 μg of either UNR or MNR Sing16 HA-OF-02, Sing16 HA-cKK-E10 or Sing16 HA-SM-102 lipid nanoparticles (LNPs). Mice were bled pre-study and 24 h post-priming. Cytokine analysis was performed using Milliplex mouse cytokine/chemokine magnetic bead panel as described in methods. Cynomolgus macaques (n = 4-6 per group) were immunized at Day 0 and Day 28 by IM route with 135 μg of either UNR or MNR Sing16 HA-cKK-E10 or Sing16 HA-SM-102 LNPs. Blood was taken at 6-days pre-study (baseline), and 2-days post each administration. Samples were analyzed for b serum cytokine levels by Luminex and c whole blood transcriptomic analysis; volcano plots were made for select cytokines/chemokines of interest.

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