Fig. 2: Bulk cell densities and growth rates.

A Average density (cells/mL) in function of time (days) for the bacterial bulk community in loop 1 (16°, orange), loop 2 (20 °C, green) and loop 3 (24 °C, blue). Per timepoint, biological replicates (n = 3) were taken (n_{Loop 1} = 363, n_{Loop 2} = 364, n_{Loop 3} = 364), corresponding error bars are shown in black. Temperature had a significant influence on bulk cell densities (p < 0.05, Kruskal-Wallis test). B Specific growth rate (h⁻¹) per loop, no significant influence of temperature on growth rate was observed over the duration of the experiment (p_{Loop 1} = 0.59, p_{Loop 2} = 0.59, p_{Loop 3} = 0.9, Mann–Whitney-test). C Specific growth rate (h⁻¹) and (D) carrying capacity (cells/mL) for treated groundwater (green) and surface water (blue) per loop. Jitter plots and boxplots are used to represent the data in Fig. 2B–D. Jitters of loop 1 (16), loop 2 (20 °C) and loop 3 (24°) are represented with orange, green and blue dots, respectively. The black points are the outliers for the boxplot calculation. Statistics are indicated using ‘ns’: not significant, ‘*’: p < 0.01, ‘**’: p < 0.001 (Mann–Whitney-test).