Fig. 5: NMDS of bulk and biofilm composition.

NMDS analysis of the relative bacterial community composition of bulk (▲) and biofilm (●) samples of loop 1 (16°, orange), loop 2 (20 °C, green) and loop 3 (24 °C, blue). Timepoints are indicated above each shape. K-means clustering resulted in three clusters, indicated with gray ellipses (95% confidence interval) and the corresponding average distance within each cluster was 0.50 for the biofilm cluster, 0.86 for the bulk cluster and 0.76 for the bulk/biofilm cluster (day 0, 14). This indicates higher similarity between the biofilm samples suggesting maturity in the community composition. Common ASVs in each group are shown in gray, and ASVs present in multiple clusters are shown in red. Corresponding taxa can be found in the Supplementary Table 2.