Extended Data Fig. 4: Quantification of 26-plex profiling for 9 PPIs and 8 signalling and organelle markers in HCC827. | Nature Biomedical Engineering

Extended Data Fig. 4: Quantification of 26-plex profiling for 9 PPIs and 8 signalling and organelle markers in HCC827.

From: Spatially resolved subcellular protein–protein interactomics in drug-perturbed lung-cancer cultures and tissues

Extended Data Fig. 4

a, The comparison of PPI counts in HCC827 whole cells, nuclei, and cytosol between those treated with and without Osimertinib. Statistical testing was performed using Mann Whitney Wilcoxon Test two-sided (***: 0.0001 < p <= 0.001, ****: p < =0.0001). The total cell numbers are 836 and 655 for untreated and Osimertinib-treated cells in the comparison of PPI count per cell. b, UMAP visualizes the similarity of p-ERK/c-Myc counts in the 5PPI and 9PPI datasets. Box plots: median (horizontal line inside box), 25th and 75th percentiles (box), 25th and 75th percentiles ±1.5 times the interquartile range (whiskers).

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