Extended Data Fig. 1: Evaluation and quantification of iseqPLA properties in HCC827 cells.
a, Visualization of the workflow evaluating PLA sensitivity, specificity, baseline vs PPI, and batch consistency in HCC827 cells. Following different treatments, the cells were stained with PLA or IF. The detailed experimental designs are in Supplementary Fig. 6a, 7a, 8a, and 9a, Created with BioRender.com. b, The comparison of PPI counts in HCC827 cells between those treated with a range of Osimertinib for 12 hours. The detailed results are in Supplementary Fig. 6. c, The comparison of PPI counts in two HCC827AR cells. The detailed results are in Supplementary Fig. 7. d, The comparison of PPI counts in HCC827 cells between those treated with and without Osimertinib. The baseline levels of 4 proteins in HCC827 cells with and without treatment were quantified on the right panel. The detailed results are in Supplementary Fig. 8. e, The comparison of PPI counts from 4 pairs in HCC827 cells from two batches treated with and without 12-hour Osimertinib. The detailed results are in Supplementary Fig. 9. Statistical testing was performed using Mann Whitney Wilcoxon Test two-sided (***: 0.0001 < p <= 0.001, ****: p < =0.0001). Box plots and violin plots: median (horizontal line inside box), 25th and 75th percentiles (box), 25th and 75th percentiles ±1.5 times the interquartile range (whiskers).
