Extended Data Fig. 4: Investigation of optimal ultrasonic antimicrobial conditions for FFRK8 peptide.
From: A sonosensitive diphenylalanine-based broad-spectrum antimicrobial peptide
Antibacterial experiments on MRSA using different concentrations of FFRK8 under US. The experimental results of spread-plate assay are respectively presented in the form of antibacterial efficiency (a) and counting of CFU/mL (b). c, After undergoing 15 min of antibacterial treatment with FFRK8+US, the supernatant was either removed by centrifugation or left intact, and then the treated MRSA were cultured in a 96-well plate. After 24 h of incubation, the absorbance at 600 nm was measured. n = 6. d, Antibacterial ability of FFRK8 with US treatment for 15 and 30 min, respectively. Panels b and d are shown as mean ± s.d. n = 5 biological replicates. Statistical significance was calculated using one- (b) or two-way (d) ANOVA.
