Extended Data Fig. 5: Biocompatibility testing of peptides.
From: A sonosensitive diphenylalanine-based broad-spectrum antimicrobial peptide
a, Representative live/dead fluorescence staining of HAMA hydrogel loaded BMSCs and paravertebral fibroblasts treated with FFRK8, US, and FFRK8+US for 15 min. Green fluorescence stained by Calcein dye indicates live BMSCs and paravertebral fibroblasts cells, and red fluorescence stained by PI dye represents dead cells. b,c, Mean fluorescence intensity (MFI) visually quantifies the green and red fluorescence intensity (arbitrary units; a.u.) of BMSCs and paravertebral fibroblasts. ns: no significant. The values are presented as mean ± s.d. of n = 3 biological replicates, and were calculated using two-way ANOVA. d,e, Testing of IC50 of FFRK8 peptide on BMSCs and paravertebral fibroblasts. Cell viability of BMSCs and paravertebral fibroblasts treated by FFRK8. Data and error bars represent the mean ± s.d., with n = 3 biological replicates.
