Extended Data Fig. 2: AAVp2CV-FGF12 gene transfer enhances the phosphorylation of MEF2a and the expression of its downstream target genes in PAH mice.
Mice received an intratracheal injection of either saline or AAV and were exposed to chronic hypoxia and weekly injection of SU5416 (Hyp+ SU) for 3 weeks for PAH induction. Mice maintained under normoxia served as the normal control. (a) Protein levels of p-MEF2a, MEF2a, and FGF12 in lung tissues as determined by western blot analysis. The band intensities of p-MEF2a were normalized to those of MEF2a and expressed relative to those of the normal controls (n = 4 biological replicates). (b) mRNA levels of MEF2a target genes related to the cell cycle (Ccne1, Mcm6) and contractile SMC markers (Lmod1, Myocd) as evaluated by real-time RT‒PCR (n = 3–4 biological replicates). All data are presented as the mean ± SEM. (one-way ANOVA with Bonferroni post hoc analysis).
