Extended Data Fig. 1: Directed differentiation and characterization of iLSEP.
From: Self-organization of sinusoidal vessels in pluripotent stem cell-derived human liver bud organoids
a) Time-lapse morphological representation of differentiation of iLSEP in micropatterned colony. Red arrows indicate expanding CD34+ endothelial regions (together with Extended Data Fig. 1b). b) Immunofluorescent staining for CD34 and Brachyury at day6. c) Immunofluorescent staining for CD31 and CD32b at day6. d) Scatter dot-plot of the flow cytometry analysis of CD31/CD32 expression in CD34+CD43− iLSEP and iAEC after CD34+ purification at day 8. CD43 is a marker to exclude fractions of the hematopoietic population. e) Heatmap of iPSC, iSTM, conventional EC (iAEC), iLSEP, and primary LSEC showing lineage marker gene set. Mes.: Mesenchyme; Endo.: pan-endothelium; LSEC: liver sinusoidal endothelial cell. f) Scatter dot-plot of the flow cytometry analysis of CD31/CD32 expression in CD34+CD43− VA at day8.
