Extended Data Fig. 6: Dental mesenchyme induction from mdEM of the proximal-oral domain.
From: Jawbone-like organoids generated from human pluripotent stem cells
a, Bright-field image and immunofluorescence staining for TFAP2B and LHX6, TFAP2A and PAX9 in day 17 aggregates after dental mesenchyme induction. Nuclei stained with DAPI. Dashed boxes magnified to the right. (n = 3 from three biologically independent experiments). Representative data are shown. Scale bars, 100 µm. b,c, RNA-seq analysis of NCC aggregates (d5) and dental mesenchyme (d17). b, Volcano plot for differential expression genes. Some dental mesenchyme markers were selected in the plot. (n = 3 from three biologically independent experiments). c, Gene-Set Enrichment Analysis (GSEA). Top 15 enriched (activated and suppressed) pathways according to GO terms for biological processes shown. False discovery rate (FDR)–adjusted P-values (adj. P-value) (one-tailed) are calculated using the Benjamini-Hochberg method. adj. P-value < 0.05. (n = 3 from three biologically independent experiments). NES, normalized enrichment score.
