Extended Data Fig. 6: Investigation of humoral immune responses and memory B cells elicited by various LNPs. | Nature Biomedical Engineering

Extended Data Fig. 6: Investigation of humoral immune responses and memory B cells elicited by various LNPs.

From: Mannich reaction-based combinatorial libraries identify antioxidant ionizable lipids for mRNA delivery with reduced immunogenicity

Extended Data Fig. 6

a, Mice received intramuscular immunization with various LNP vaccines on days 0 and 21 (0.25 mg/kg). IgG2c to IgG1 ratio was determined. Data are presented as mean ± s.d. b, Enumeration of RBD-specific B cells per spleen. Splenocytes were stimulated with SARS-CoV-2 RBD peptide pools and stained with various antibodies before flow cytometry analysis. c, Percentage of RBD-specific B cells categorized by germinal center (GC) or memory phenotype. GC B cells were defined as CD38−GL7+, and memory B cells were defined as CD38+GL7−. Data are shown as mean ± SD (n = 5). Statistical differences were calculated using one-way ANOVA with Tukey’s post hoc test.

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