Extended Data Fig. 2: C-a16 LNPs induce antigen-specific immune responses.
a-d, Quantification of LNP distribution in immune cell populations, including DCs (a), macrophages (b), B cells (c), and T cells (d), assessed by flow cytometry. Data are presented as mean ± SD (n = 5), and statistical differences were determined by one-way ANOVA. e, Immunofluorescence images of lymph nodes in mice after intramuscular injection of DiR dye-labeled LNPs. Nuclei are represented in blue, CD11c+ DCs in green, and DiR-labeled LNPs in red. f, Evaluation of OVA-specific T cell generation following two doses of C-a16-mOVA vaccines. Seven days after the boost dose, blood T cells were stained with SIINFEKL-H2Kb tetramer-APC antibody. After that, SIINFEKL-H2Kb tetramer+ cells were detected by flow cytometry (f) and quantified (g). h and i, Investigation of vaccine-induced immune responses’ ability to eliminate SIINFEKL epitope+ target cells. A mixture of CFSElow SIINFEKL-loaded and CFSEhigh non-loaded splenocytes (in a 1:1 ratio) was intravenously injected into vaccinated mice. After 24 hours, splenocytes were collected and analyzed by flow cytometry (h), and target cell killing was quantified (i). Data in a-d, g and i are shown as mean ± SD (n = 5). Statistical differences were calculated using one-way ANOVA with Tukey’s post hoc test.
