Extended Data Fig. 4: T cell immune responses elicited by C-a16 LNP encapsulating mRNA encoding SARS-CoV-2 spike protein.
Mice received intramuscular immunization with various LNP vaccines on days 0 and 21 (0.25 mg/kg). On day 35, splenocytes were collected and stimulated with SARS-CoV-2 RBD peptide pools. a and b, T cells were assessed for Th2 (IL-4, IL-5) and c, Th17 (IL-17a) intracellular cytokine expression. d, Evaluation of the expression of the cytotoxic marker CD107α expression in CD8+ T cells. Data are shown as mean ± SD (n = 5). Statistical differences were calculated using one-way ANOVA with Tukey’s post hoc test.
