Extended Data Fig. 6: Cortical layer marker expression and neuronal morphology in XG-hCO.
From: Scalable production of human cortical organoids using a biocompatible polymer
(a) Immunostaining for CTIP2 (magenta) and Hoechst (cyan) at day 62 (left panels, n = 3 for hCO and n = 4 for XG-hXO), GFAP (green), MAP2 (magenta) and Hoechst (cyan) at day 100 (middle panels, n = 8 for hCO and n = 6 for XG-hXO), SATB2 (magenta) and Hoechst (cyan) at day 100 (right panels, n = 6 for hCO and n = 6 for XG-hXO) in hCO and XG-hCO. Scale bars: 100 µm for left and middle panels, and 200 µm for right panels. (b) Confocal live Imaging of virally labeled eYFP+ neurons in hCO and XG-hCO. Representative images of neurons expressing eYFP in hCO and hCO + XG. Quantification of soma area, soma circularity and the number of neurites. n = 95 neurons from 5 organoids for hCO, n = 100 neurons from 6 organoids for hCO + XG from 2 differentiation experiments of 2 hiPS cell lines. Two-tailed Mann-Whitney test, P = 0.86 for soma area, P = 0.52 for soma circularity, and P = 0.15 for the number of neurites. Data show mean ± s.e.m.
