Fig. 2: ASTRA results detected in the HIV-affected Eswatini cohort.
From: Self-powered rapid antigen-specific T-cell response assay for Mycobacterium tuberculosis infections
a, Eswatini cohort (n = 44) HIV status, microbiologic (Xpert, smear, culture) and clinical (chest X-ray and NIH-defined symptoms) evidence of TB, immunologic evidence of M.tb infection (IGRA) and ASTRA 4-1BB + OX-40 results. b,c, M.tb-peptide-stimulated ASTRA 4-1BB + OX-40 and IFNγ PBMC responses (positive-cell percentages) for (b) HIV-positive and -negative TB cases (n = 18 biological replicates) and (c) individuals with LTBI or no evidence of M.tb infection (non-M.tb controls) (n = 24 biological replicates) d,e, M.tb-peptide-stimulated ASTRA 4-1BB + OX-40 results detected with PMBCs from Eswatini participants with (d) TB (n = 20 biological replicates) and (e) LTBI with and without HIV-1 co-infection (n = 10 biological replicates). Bar graphs display mean ± s.d. Violin plots depict the distribution of data density (outline), the median (dashed line) and the first and third interquartile boundaries (dotted lines). Dashed red lines indicates the lower threshold for positive signal. **P < 0.01, ***P < 0.001, ****P < 0.0001; two-way ANOVA among ASTRA 4-1BB/OX-40 and ASTRA IFNγ.
