Extended Data Fig. 6: Additionally verifying the expression of EGFR28ΞΆ-CARs and EGFR-BiKEs. | Nature Biomedical Engineering

Extended Data Fig. 6: Additionally verifying the expression of EGFR28ΞΆ-CARs and EGFR-BiKEs.

From: Bispecific killer cell engager-secreting CAR-T cells redirect natural killer specificity to enhance antitumour responses

Extended Data Fig. 6

a. Surface CAR expression of HEK293T cells transfected with plasmid vectors as indicated, examined by flow cytometry. b. Coomassie Blue staining of SDS-PAGE gels to detect EGFR-BiKEs secreted by HEK293T cells transfected with plasmid vectors as indicated. Unconc, unconcentrated. Conc, concentrated. c. Immunoblotting identification of EGFR-BiKEs secreted by HEK293T cells transfected with EGFR28ΞΆ BiKEβˆ’ or BiKE+ plasmid vectors. d. Binding traits of EGFR-BiKEs secreted by HEK293T cells transfected with EGFR28ΞΆ BiKE+ plasmid vectors towards SK-OV-3 cells and NK cells, examined through flow cytometry. SN, supernatant. e. Binding traits of secreted BiKEs towards T cells as a negative control, examined through flow cytometry. SN, supernatant. f. Statistics on the surface expression of CAR (represented as MFI) for EGFR28ΞΆ BiKEβˆ’ or BiKE+ CAR-T cells generated from three independent donors. Paired, two-tailed Student’s t-tests were performed. g. Retroviral transduction efficiency in the production of EGFR28ΞΆ BiKEβˆ’ or BiKE+ CAR-T cells from two other donors.

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