Extended Data Fig. 9: Additionally verifying the expression of 1928ΞΆ-CARs and EGFR-BiKEs.
a. Surface CAR expression of HEK293T cells transfected with plasmid vectors as indicated, examined by flow cytometry. b. Coomassie Blue staining of SDS-PAGE gels to detect EGFR-BiKEs secreted by HEK293T cells transfected with plasmid vectors as indicated. Unconc, unconcentrated. Conc, concentrated. c. Immunoblotting identification of EGFR-BiKEs secreted by HEK293T cells transfected with 1928ΞΆ BiKEβ or BiKE+ plasmid vectors. d. Binding traits of EGFR-BiKEs secreted by HEK293T cells transfected with 1928ΞΆ BiKE+ plasmid vectors towards SK-OV-3 cells and NK cells, examined through flow cytometry. SN, supernatant. e. Retroviral transduction efficiency in the production of 1928ΞΆ BiKEβ or BiKE+ CAR-T cells from three donors. f. Statistics on the surface expression of CAR (represented as MFI) for 1928ΞΆ BiKEβ or BiKE+ CAR-T cells generated from three independent donors. Paired, two-tailed Studentβs t-tests were performed. g. Detection of EGFR-BiKEs secreted by 1928ΞΆ BiKEβ or BiKE+ CAR-T cells at indicated time points after transduction. CAR-T cells from independent donors (nβ=β3) were sampled. Unpaired, two-tailed multiple t-tests were performed and data are presented as mean valuesβΒ±βSD.
