Extended Data Fig. 2: Additionally testing the in vitro properties and functions of 1928ΞΆ CAR-T and CAR-NK cells and redirection of NK cells. | Nature Biomedical Engineering

Extended Data Fig. 2: Additionally testing the in vitro properties and functions of 1928ΞΆ CAR-T and CAR-NK cells and redirection of NK cells.

From: Bispecific killer cell engager-secreting CAR-T cells redirect natural killer specificity to enhance antitumour responses

Extended Data Fig. 2

a. Histograms for extra two biological replicates of CFSE assays showing the percentage of proliferated 1928ΞΆ BiKEβˆ’ or BiKE+ CAR-T cells (top) and 1928ΞΆ BiTEβˆ’ or BiTE+ CAR-NK cells (bottom) with or without antigen stimulation. b. Percentage of lysed NALM6 cells after 16 h coculture with primary NK cells at indicated E:T ratios in supernatant collected from 1928ΞΆ BiKEβˆ’ or BiKE+ CAR-T cells. SN, supernatant. NK cells from independent donors (n = 3) were sampled. Unpaired, two-tailed multiple t-tests were performed and data are presented as mean values ± SD. P-values greater than 0.05 are not shown. c. Histograms showing the surface expression of three exhaustion markers on 1928ΞΆ BiKEβˆ’ or BiKE+ CAR-T cells of extra two biological replicates with or without consistent antigen stimulation. d. The concentration of CD19-BiKEs secreted by equal amounts of 1928ΞΆ BiKE+ CAR-T cells with or without consistent antigen stimulation. CAR-T cells from independent donors (n = 3) were sampled. Unpaired, two-tailed Student’s t-tests were performed and data are presented as mean values ± SD.

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