Extended Data Fig. 2: The γ-globin expression was rapidly activated following base editing at the sgRNA-25 target site within the HBG promoter.

a. Schematic diagram of the sequence of the γ-globin promoter of CD34⁺ promoter editing cells. The left arrow indicates the sequence position of sgRNA-25, and the two upward arrows indicate the targeted bases. b. ATAC-seq of in vitro differentiation of ABE8e RNP edited and un-edited CD34⁺ cells. c. SP1 CUT& Tag profiles in the β-globin cluster. Antibodies and cell types for each track are shown on the right. The promoters of duplicated γ-globin genes (HBG2 and HBG1) are highlighted in gray. d. Zoomed-in view of the HBG2 and HBG1 promoter regions. The sequence of sgRNA-25 is highlighted in orange. Heatmap comparison of signals in primary human CD34⁺-derived erythroid cells with or without sgRNA-25 base editing within binding sites. e. The enrichment curve of the CUT&Tag binding signal in the upstream and downstream 3-kb regions of the gene body and TSS region. f. Heat map comparison of overlapping peaks CUT&TAG.