Extended Data Fig. 4: Characterization of the adenovirus carrying BA.5 spike protein (Ad5BA.5) and recombinant trimeric RBDBA.5-HR protein.
a, The schematic representation of the design of full-length BA.5 spike protein-carrying adenovirus (top). Western blotting of spike protein expression in A549 cells in the absence or presence of Ad5BA.5 (bottom). SP signal peptide, NTD N-terminal domain, RBD receptor binding domain, HR1 and HR2 heptad repeats 1 and 2, TM transmembrane domain, CP cytoplasmic domain. b, RBDBA.5-HR protein includes RBD derived from the BA.5 variant and HR1 and HR2 domains in subunit S2 of the spike protein. The representative elution chromatographs of the recombinant RBDBA.5-HR protein using a calibrated Superdex 200 Increase column (bottom right). The SDS-PAGE analysis of the eluted protein is shown. M, marker; 1-3, the eluted samples of the ascending part and descending part of the peak. a-b are representative of two independent experiments with similar results. c, The schematic representations of the preparation of Ad5BA.5 plus RBDBA.5-HR. Schematic illustrations in a-c were created in BioRender. Wang, Y. (2025) https://BioRender.com/1jokmtk.
