Extended Data Fig. 6: Hypoxia promotes the accumulation of pyrimidine precursors.
From: De novo pyrimidine biosynthetic complexes support cancer cell proliferation and ferroptosis defence
a, Fractional labeling of carbamoyl aspartate, dihydroorotate, orotate, and UMP in Hela cells cultured in medium containing 1 mM 13C5-glutamine with or without uridine (400 μM) supplementation under normoxia or hypoxia for 8 h. b-d, Labeled fractions of carbamoyl aspartate, dihydroorotate, orotate and UMP (b), relative intracellular levels (c) and fractional labeling of newly synthesized carbamoyl aspartate, dihydroorotate, orotate and UMP (d) in HEK293T cells cultured under normoxia or hypoxia for 8 h with 1 mM 13C5-glutamine. e, Volcano plot of the differential metabolites between hypoxia group and normoxia group. HeLa cells were cultured under normoxia or hypoxia for 8 h. (a-d) Values are shown as the mean ± SD, n = 3 biologically independent samples. P values were obtained using one-way ANOVA with Tukey test (a) and unpaired two-tailed Student’s t-test (b-e).
