Extended Data Fig. 2: The enzymes involved in pyrimidine biosynthesis form the multi-enzyme complex.
From: De novo pyrimidine biosynthetic complexes support cancer cell proliferation and ferroptosis defence
a, HEK293T cells expressing Flag-CAD were subjected to IP analysis, followed by immunoblotting. Flag-vector was used as a negative control. b, IP analysis of the interaction between endogenous CAD, DHODH, UMPS and GOT1 in HEK293T cells with anti-UMPS antibody. Rabbit IgG was used as a negative control. c, HEK293T cells expressing Flag-UMPS were subjected to IP analysis, followed by immunoblotting. d, HEK293T cells expressing Flag-tagged truncations of GOT1 were subjected to IP analysis using anti-Flag M2 beads, followed by immunoblotting. N-terminal region, 1-214aa; C-terminal region, 215-413aa. e, HEK293T cells expressing truncations of UMPS were subjected to IP analysis using anti-Flag M2 beads, followed by immunoblotting. f, Schematic showing the interaction between CAD, GOT1 and UMPS to promote the incorporation of newly synthesized aspartate into pyrimidine. (a-e) Data are verified in three replicates with similar results.
