Extended Data Fig. 6: Modelling vessel co-option in vitro.
(a) Device immunostaining of HMBEC lifeact-Ruby (magenta), 1205Lu lifeact-GFP (green) and nuclei (hoechst, blue). From left to right, representative images of a scramble control, a talin 1 KO, a talin 2 KO and a talin 1 and 2 KO cell adhering to the micro-vessel. Maximum projection, scale bar 20µm. (b) Cell sphericity of scramble control, talin 1 KO, talin 2 KO and talin 1 and 2 KO cells adhered to the micro-vessel (Scr: n=53, 7 devices; Tln1 KO: n=25, 6 devices; Tln2 KO: n=42, 6 devices; Tln1+Tln2 KO: n=29, 8 devices; 3 experiments). Lighter dots represent each cell value and darker dots represent the median value per device. Statistical analysis: Kruskal–Wallis test followed by a post hoc Dunn’s test. Graphs show median ± interquartile range. (c) Cell velocity of scramble control and talin 1 and 2 KO cells moving along the micro-vessel (Scr: n=47, 7 devices; Tln1+Tln2 KO: n=42, 8 devices; 3 experiments). Lighter dots represent each cell value and darker dots represent the mean value per device. Statistical analysis: two-sided T-test. Graph show mean ± standard deviation. d-e, Laminin intensity (d) and cell sphericity (e) of 1205Lu cells spreading on control devices or in devices where the apoptosis of endothelial cells was triggered with the small molecule CID (Control: n=26, 7 devices; CID 5nM: n=118, 18 devices; 4 experiments). Lighter dots represent each cell value and darker dots represent the median value per device. Statistical analysis: two-sided Mann–Whitney test. Graphs show median ± interquartile range.
