Extended Data Fig. 2: Vessel co-option is a common pattern of invasion across many different cell lines.
a-c, Mean invasion depth per slice for cells adhered to vessels or to the bulk brain tissue for 1205Lu (n=7), WM983B (n=4), U2OS (n=4), MCF7 (n=6) and MDA-MB-231 (n=6) (a), A375P (n=8) and A375M2 (n=6) (b), nHDF (n=7), MCF10A (n=5) and HBVPC (n=5) cell lines (c). Full distributions shown in Fig. 1d, j–l. Data corresponds to at least 3 experiments. Statistical analysis: two-sided paired T-test or an ordinary one-way ANOVA test followed by a post hoc Tukey’s test (for b). All graphs show mean ± standard deviation. (d) Violin plots of cell shape circularities for MDA-MB-231 TGL, BrM2-831, BoM-1833 and LM2-4175 cells adhered to the vasculature. (e) Violin plots of bulk invasion depths for MDA-MB-231 TGL, BrM2-831, BoM-1833 and LM2-4174 cells. (f) Violin plots of vessel invasion depths for MDA-MB-231 TGL, BrM2-831, BoM-1833 and LM2-4175 cells. (g) Violin plots of cell shape circularities for cells adhered to vessels or to the bulk brain tissue for B16-F10, E077 and NIH-3T3 mouse-derived cell lines. (h) Violin plots of invasion depths for cells adhered to vessels or to the bulk brain tissue for B16-F10, E0771 and NIH-3T3 mouse-derived cell lines. For MDA-MB-231 TGL (parental cell line): n=394 for Bulk, n=441 for Vessel, from 5 slices, 3 experiments. For BrM2-831 (brain tropic): n=652 for Bulk, n=479 for Vessel, from 6 slices, 3 experiments. For BoM-1833 (bone tropic): n=520 for Bulk, n=496 for Vessel, from 6 slices, 3 experiments. For LM2-4175 (lung-tropic): n=564 for Bulk, n=529 for Vessel, from 6 slices, 3 experiments. For B16-F10 (melanoma): n=239 for Bulk, n=212 for Vessel, from 4 slices, 3 experiments. For E0771 (breast cancer): n=269 for Bulk, n=359 for Vessel, from 6 slices, 3 experiments. For NIH-3T3 (embryonic fibroblast): n=165 for Bulk, n=530 for Vessel, from 6 slices, 3 experiments. (i) Brain slice immunostaining of laminin (magenta) and T-cells labelled with CellTracker-488 (green). Left: z-plane 2µm below the top surface. Right: z-plane 50µm below the top surface. White insert labels zoomed area. Scale bars: 100µm, 10µm (inserts). (j) Violin plots of T-cell shape circularities when adhered to vessels or to the bulk brain tissue. (k) Violin plots of T-cell invasion depths within the slice when adhered to vessels or to the bulk brain tissue. For T-cells: n=380 for Bulk, n=208 for Vessel, from 3 slices, 2 experiments. For all violin plots, dots represent the median value for each slice analysed. Statistical analysis: two-sided Mann–Whitney or a Kruskal–Wallis test followed by a post hoc Dunn’s test (for d-f). Graphs show median ± interquartile range.
