Extended Data Fig. 4: An EM-based quantification strategy to reduce bias in quantification.
From: Real-time and programmable transcriptome sequencing with PROFIT-seq
a, Percentage of full-length reads for the target (right) and non-target (left) transcripts in the JL19 sample. b, Expression levels of the target (red) and non-target (grey) genes before EM-based correction (left) or after correction (right) in the JL19 sample. c, Correlation of target gene expression levels for Illumina total RNA-seq, control runs without pore manipulation, and PROFIT-seq data with/without EM-based quantification correction. Colors represent the correlation coefficient calculated using the spearman correlation test. Source numerical data are available in source data.
