Extended Data Fig. 5: Effective enrichment of target pathogens using PROFIT-seq.
From: Real-time and programmable transcriptome sequencing with PROFIT-seq
a, The yield of total and on-target consensus reads in control and adaptive run. The y-axis represents number of raw reads or bases of all reads (top row) or target transcripts (bottom row). Bar colors represents control and adaptive runs respectively. b, The absolute number of assigned consensus reads for each pathogen in PROFIT-seq and control runs of the 16 sputum samples. Colors represent different stages of a 6-hour window during the 24-hour run. c-d, Number of reads assigned to target pathogens during sequencing of pneumonia (c) and COVID-19 (d) samples. Colors represent PROFIT-seq (red) or control runs (blue) respectively. e, Enrichment efficiency of target pathogens in COVID-19 and pneumonia samples. f, Tracks of sequencing depth of the spike protein in the SARS-CoV-2 genome. The genome structure and spike protein domains are indicated above the tracks. Colors represent PROFIT-seq (red) or control runs (blue) respectively. g, Heatmap presentation of the S-protein variants detected in 8 COVID-19 samples. Colors indicate the number of supporting reads for each mutation. h, Composition of pathogenic microbiota in 8 sputum samples of COVID-19 patients using PROFIT-seq (upper) and control data (bottom). Source numerical data are available in source data.
