Extended Data Fig. 1: Functions of PROFIT-seq.
From: Real-time and programmable transcriptome sequencing with PROFIT-seq
a, Example of a TOML-formatted configuration file for batch job submission. b, Schematic representation of chunk data processing. c, PROFIT-seq provides flexible functions to customize the sequencing duration, channel number, demultiplexed barcode, and aligned region for each individual task. d, Length of basecalled sequences (left), mapping rate (middle), and number of reads with different end reasons (right). All molecules were set to be rejected, and runs were simulated with different chunk acquisition intervals ranging from 0.1 to 1.0 s. Colors in the right panel represent different end reasons, including adaptive sampling voltage reversal (purple), read completed (green), device changed mux (blue), read became blocked (red) and unblock voltage reversal (orange). The middle lines represent the median, and the lower and upper bounds represent the first and third quartiles, respectively. The upper and lower whiskers represent the limits of 1.5 inner quantile ranges, and points outside this range are plotted as outliers. Source numerical data are available in source data.
